The eccentric and childish Pee-wee Herman embarks on a big adventure when his beloved bicycle is stolen. Armed with information from a fortune-teller and a relentless obsession with his prized possession, Pee-wee encounters a host of odd characters and bizarre situations as he treks across the country to recover his bike.
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| Sound | Danny Elfman | Original Music Composer |
| Camera | Victor J. Kemper | Director of Photography |
| Directing | Tim Burton | Director |
| Directing | Robert Engelman | Second Assistant Director |
| Sound | Cecelia Hall | Supervising Sound Editor |
| Directing | Jan Kemper | Script Supervisor |
| Sound | David B. Cohn | Supervising Sound Editor |
| Directing | Robert P. Cohen | First Assistant Director |
| Sound | Bob Badami | Music Editor |
| Directing | Harvey Waldman | Second Assistant Director |
| Production | David Silver | Unit Production Manager |
| Directing | Willie E. Simmons, Jr. | Second Second Assistant Director |
| Crew | Marian Green | Stunts |
| Crew | Corey Michael Eubanks | Stunts |
| Crew | Fred Scheiwiller | Stunts |
| Crew | Jeff Imada | Stunts |
| Crew | Bob Herron | Stunts |
| Crew | Russell Solberg | Stunts |
| Crew | Troy Melton | Stunts |
| Crew | Angelo Lamonea | Stunts |
| Editing | Claudia Finkle | Assistant Editor |
| Sound | Roger Pietschmann | Boom Operator |
| Sound | Petur Hliddal | Production Sound Mixer |
| Sound | Denise Horta | Sound Editor |
| Camera | Mario Zavala | Second Assistant Camera |
| Sound | David J. Hudson | Sound Re-Recording Mixer |
| Sound | Neil Brody | Sound Re-Recording Mixer |
| Camera | Robert C. Thomas | Camera Operator |
| Crew | Pat Romano | Stunts |
| Sound | David Pettijohn | Sound Editor |
| Crew | J.P. Amateau | Stunts |
| Crew | Mary Albee | Stunts |
| Sound | Joseph A. Ippolito | Sound Editor |
| Sound | Terry Porter | Sound Re-Recording Mixer |
| Crew | Steve Mack | Stunts |
| Sound | Irene Bowers | Assistant Sound Editor |
| Production | Sam Mercer | Location Manager |
| Visual Effects | Chuck Gaspar | Special Effects Supervisor |
| Crew | Joe Day | Special Effects Coordinator |
| Camera | Bill Roe | First Assistant Camera |
| Art | James E. Tocci | Set Designer |
| Lighting | Patrick Marshall | Rigging Gaffer |
| Lighting | Earl Gilbert | Gaffer |
| Camera | Gaylin P. Schultz | Key Grip |
| Camera | Bernie Schwartz | Dolly Grip |
| Camera | Audie Aragon | Best Boy Grip |
| Art | Margie Stone McShirley | Assistant Art Director |
| Crew | Paul Baxley | Stunt Coordinator |
| Art | David Q. Quick | Assistant Property Master |
| Costume & Make-Up | Don Vargas | Costumer |
| Costume & Make-Up | Frank Griffin | Makeup Artist |
| Editing | Donah Bassett | Negative Cutter |
| Art | Bruce J. Gfeller | Construction Coordinator |
| Crew | Jeff Freedman | Unit Publicist |
| Crew | Mario Simon | Transportation Captain |
| Crew | Randy Peters | Transportation Coordinator |
| Editing | Richard Ritchie | Color Timer |
| Costume & Make-Up | Sandy Berke Jordan | Costumer |
| Crew | Tino Caira | Transportation Captain |
| Camera | Peter Sorel | Still Photographer |
| Art | Steven M. Levine | Property Master |
| Art | Anthony Goldschmidt | Title Designer |
| Costume & Make-Up | Linda Trainoff | Hairstylist |
| Production | William E. McEuen | Executive Producer |
| Production | Robert Shapiro | Producer |
| Production | Richard Gilbert Abramson | Producer |
| Editing | Billy Weber | Editor |
| Production | Wallis Nicita | Casting |
| Crew | Steven Lambert | Stunt Double |
| Writing | Phil Hartman | Writer |
| Writing | Paul Reubens | Writer |
| Writing | Michael Varhol | Writer |
| Art | David L. Snyder | Production Design |
| Art | David L. Snyder | Art Direction |
| Art | Paul Reubens | Set Decoration |
| Art | Thomas L. Roysden | Set Decoration |
| Costume & Make-Up | Aggie Guerard Rodgers | Costume Design |
| Crew | Eurlyne Epper | Stunts |
| Crew | Solly Marx | Stunts |
